Up to three S1000 cyclers can be connected to a C1000 Touch cycler, and the four cyclers can then be conveniently controlled as one high-throughput instrument. The cycler can be used as a stand-alone instrument for PCR, or it can be connected to a C1000 Touch™ thermal cycler for additional throughput. When importing plate setup information from a plate setup file, the software overwrites all existing settings with the information in the file.The S1000 thermal cycler combines powerful performance with streamlined features. If the samples and/or targets that you include in the plate setup file are present in other experiments included in the project, the names in the file must match those in the other experiments exactly (including case) in order for the software to associate the data. For example, on a 96-well plate, the number of well A1 is "0" and the number of well G12 is "95".Ĭolumn B (Well Position) – The coordinates of the well on the plate.Ĭolumn C (Sample Name) – The name of the sample in the well (up to 256-characters).Ĭolumn D (Task) – The task of the sample in the well, where acceptable values include UNKNOWN, NTC, and your custom names for positive controls.Ĭolumn F (Reporter) – The name of the reporter dye present in the well.Ĭolumn G (Quencher) – The name of the quencher dye present in the well. As shown in the example above, the rows can occur in any order, but the location information (in columns 1 and 2) must be accurate.įor each well the file contains the following information:Ĭolumn A (Well) – The numerical position of the well on the plate, where wells are numbered left to right and top to bottom.
Rows 7 and below contain the plate setup information for the experiment, where each row contains the information for the contents of a single well on the reaction plate. Enter the headings exactly as shown, including upper- and lowercase letters: In general, do not edit this information as it is identical for all files that you use. Rows 1 to 6 contain file header information that describes the experiment. Use the following guidelines when editing the file:
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